The immune system plays a major role in maintaining many physiological processes in the reproductive system. The current annotation count on this page is being calculated. The technology necessary to execute our method is readily available in most biological laboratories, does not require robotic or microfluidic devices, and dramatically reduces resource needs and resulting costs of the traditional high-throughput studies. We also developed segmentation-free image analysis capable of handling large optical fields containing thousands of cells in the sample, even in confluent growth condition. To further application of this technology, we developed a fluorescent barcodingmethod that does not require any chemical drug(s) modifications. We use these data for statistical prediction of the treatment response to the drugs in a macroscopic population of cells. After the incubation period, the ensuing phenotype and corresponding drug barcodes for each cell are recorded.

We create independent transient drug gradients across the cell culture sample to produce heterogeneous local drug combinations. A single sample containing thousands of cells is treated with a combination of fluorescently barcoded drugs. We rely on the randomness of drug uptake in individual cells as a tool to create and encode drug treatment regimens. We develop a method that allows one to test a large number of drug combinations in a single cell culture sample.

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